2 4 Iodophenyl 3 2 4 Dinitrophenyl 5 2 4 Disulfophenyl 2h Tetrazolium Sodium Salt

2-% 284-iodophenyl% 29-3-% 282% 2C4-dinitrophenyl% 29-5-% 282% 2C4-disulfonylphenyl% 29-2H-tetrazole, sodium salt, this is an organic compound with a particularly complex structure. According to its naming, its structure can be gradually analyzed.
"2- (4-iodophenyl) ", that is, the No. 2 position of the tetrazole ring, is connected to an iodine-containing phenyl group. Iodine atoms have unique chemical properties, which affect the reactivity and physical properties of compounds.
"3- (2,4-dinitrophenyl) ", indicating that at position 3 of the tetrazole ring, there is a 2,4-dinitro-substituted phenyl group. The nitro group is a strong electron-absorbing group, which reduces the electron cloud density of the phenyl group and further affects the electron distribution and chemical activity of the whole molecule.
"5- (2,4-disulfonylphenyl) ", which means that at position 5 of the tetrazole ring, there is a 2,4-disulfonyl-substituted phenyl group. The sulfonic acid group is a strong hydrophilic group, giving the compound good water solubility.
The sodium salt form shows that the compound binds to sodium ions. The presence of sodium ions alters the solubility and ionic properties of the compound. Overall, different substituents in the structure of this compound give it a variety of chemical and physical properties, which may have specific uses in the fields of organic synthesis and biochemical research.

2-% 284-iodophenyl% 29-3-% 282% 2C4-dinitrophenyl% 29-5-% 282% 2C4-disulfonate phenyl% 29-2H-tetrazole, sodium salt, which is commonly known as WST-1 reagent. This substance has a wide range of uses and is important in the field of biological and medical experiments.
First, it is used for the detection of cell proliferation and cytotoxicity. During cell proliferation, dehydrogenase in the mitochondria of living cells can reduce WST-1 reagent to water-soluble formin products. The more vigorous the cell proliferation, the higher the dehydrogenase activity, and the more formin products are generated. By measuring the absorbance, the cell proliferation status can be sensitively reflected. Similarly, if a cell is exposed to toxic substances, the cell viability decreases, the activity of dehydrogenase decreases, and the amount of nail production decreases, the cytotoxicity of the substance can be evaluated.
Second, for drug screening. In the process of new drug development, it is necessary to evaluate the activity of a large number of potential drugs at the cell level. By detecting the effect of drugs on cell proliferation or toxicity with the help of WST-1 reagent, it can quickly filter out drug compounds with potential activity, which lays the foundation for subsequent in-depth research.
Third, for immune cell activity analysis. In immunological research, the activation and proliferation state of immune cells such as T cells and B cells are related to the intensity and direction of the immune response. The use of WST-1 reagent can accurately determine the proliferation of immune cells under different stimulation conditions, and help to explore the mechanism of immune regulation.
Fourth, it is used for apoptosis research. During apoptosis, mitochondrial function is damaged and dehydrogenase activity is changed. WST-1 reagent is sensitive to changes in cell mitochondrial function and can indirectly reflect the occurrence of apoptosis, providing a powerful tool for studying the mechanism of apoptosis.
This reagent plays an indispensable role in many aspects of life science research due to its convenience, sensitivity and accuracy.

2-% 284-iodophenyl% 29-3-% 282% 2C4-dinitrophenyl% 29-5-% 282% 2C4-disulfonic acid phenyl% 29-2H-tetrazolium, sodium salt, often referred to as Fuchsin tetrazolium salt, mostly used in biochemical research, its usage is as follows:
In the experiment of cell viability and proliferation detection, dissolve this reagent in an appropriate amount of buffer to prepare a suitable concentration working solution. Usually, according to the needs of the experiment, the configuration concentration is between 0.1 and 1 mg/mL. During the experiment, remove the cell culture medium, gently rinse the cells with PBS, then add the working solution containing the reagent to each well, and incubate for a specific time in the cell culture box at a suitable temperature (usually 37 ° C), usually 1-4 hours. Because the mitochondrial dehydrogenase of living cells can reduce this reagent to form a red nail product, the absorbance of the specific wavelength (about 490-570 nm) of the enzyme labeling machine can be determined according to the absorbance value. Cell viability and proliferation status can be judged according to the absorbance value.
Stain plant tissue, take fresh plant tissue sections or sections, immerse in the working solution containing the reagent, and incubate under suitable light and temperature conditions. After several hours to several days, it varies according to tissue type and reaction status. At the end of staining, rinse the tissue with buffer solution to remove unreacted reagents. Plant living cells develop color due to reducible reagents, according to which physiological characteristics such as metabolic activity and respiratory effect check point of plant tissue can be observed.
When using, be sure to follow laboratory safety regulations, because the reagents contain iodine, nitro and other groups, or have potential toxicity and irritation. Proper protection to avoid contact with skin and eyes. And now use and prepare to ensure the activity of the reagents and obtain accurate experimental results.

2-% 284-iodophenyl% 29-3-% 282% 2C4-dinitrophenyl% 29-5-% 282% 2C4-disulfonylphenyl% 29-2H-tetrazolium, sodium salt, the storage conditions of this chemical reagent are related to its quality and effectiveness, which cannot be ignored.
This reagent should be stored in a cool place, because the temperature is too high, or its chemical structure changes, and it loses its original properties. And should be placed in a dry place to avoid moisture. If moisture invades, it may cause a chemical reaction and cause the reagent to deteriorate.
The place where it is stored needs to be shaded from light. The damage of light may cause the reagent to undergo photochemical reactions, which will damage its purity and activity.
Furthermore, it must be sealed and stored. First, to prevent it from contacting with oxygen, carbon dioxide and other gases in the air, causing changes in composition; second, to avoid dust and impurities from mixing in to keep it pure.
After taking it, seal it as it was before and place it in the designated storage place to protect its quality. These are all important principles for proper storage of the reagent. Follow them to ensure that the reagent can be used without worry.

2-% 284-Iodophenyl% 29-3-% 282% 2C4-dinitrophenyl% 29-5-% 282% 2C4-disulfonylphenyl% 29-2H-tetrazole, sodium salt, this chemical is related to safety and requires detailed inspection.
The appearance of this substance is often a solid powder, and the color may vary. In the laboratory, if it is not handled properly, the powder may cause risk of inhalation. It can irritate the respiratory tract, causing cough and respiratory discomfort. Contact with the skin, or cause allergies, irritation, redness, swelling, and itching. Accidentally entering the eyes is particularly harmful, which can damage eye tissue and affect vision.
From the chemical properties point of view, in case of strong oxidizing agent, or a violent reaction, there is a risk of explosion. When storing, the temperature and humidity have a great impact, high temperature, humid environment or cause it to deteriorate, the stability is reduced, and the safety is also reduced.
When using this object, the protection must be comprehensive. Experimenters should wear protective clothing, protective gloves, goggles, and operate in a well-ventilated place to prevent inhalation and contact. Storage should be in a cool, dry, and well-ventilated place, away from oxidants and fire sources. Dispose of waste according to regulations, do not discard it at will, and avoid polluting the environment.
In summary, 2-% 284-iodophenyl% 29-3-% 282% 2C4-dinitrophenyl% 29-5-% 282% 2C4-disulfonylphenyl% 29-2H-tetrazole, sodium salts are safe or not, depending on the operation, storage, and disposal laws and regulations, and can be guaranteed to be safe.