2-(4-Iodophenyl)-3-(4-Nitrophenyl)-5-(2,4-Disulfophenyl)-2H-Tetrazolium,Monosodium Salt

Fengxi Chemical

Specifications

HS Code	536880
Chemical Formula	C20H13IN3NaO10S2
Molecular Weight	695.36 g/mol
Appearance	reddish - brown powder
Solubility	Soluble in water
Ph Stability	Stable over a certain pH range
Storage Condition	Stored at -20°C, protected from light
Use	Used in biochemical assays for cell viability
Assay Principle	Reduction by mitochondrial dehydrogenases
Color Change	Changes from red - brown to formazan (colored product)
Sensitivity	High sensitivity for detecting viable cells
Chemical Formula	C20H13IN4Na2O10S2
Molecular Weight	725.36 g/mol
Appearance	reddish - brown powder
Solubility	Soluble in water
Purity	Typically high purity, e.g., ≥98%
Ph Stability	Stable within a certain pH range
Spectral Properties	Absorbance maxima at specific wavelengths
Storage Conditions	Stored at -20°C, protected from light
Reactivity	Reacts with reducing agents
Application	Used in cell viability assays
Chemical Name	2-(4-Iodophenyl)-3-(4-Nitrophenyl)-5-(2,4-Disulfophenyl)-2H-Tetrazolium, Monosodium Salt
Molecular Formula	C19H12IN3Na2O8S2
Molecular Weight	661.34 g/mol
Appearance	reddish-brown powder
Solubility	Soluble in water
Ph	5.5 - 7.5 (1% solution)
Storage Conditions	Store at -20°C
Purity	Typically >95%
Application	Used in cell viability assays
Cas Number	14649-16-4
Melting Point	Decomposes

As an accredited 2-(4-Iodophenyl)-3-(4-Nitrophenyl)-5-(2,4-Disulfophenyl)-2H-Tetrazolium,Monosodium Salt factory, we enforce strict quality protocols—every batch undergoes rigorous testing to ensure consistent efficacy and safety standards.

Packing & Storage

Packing	1 vial containing [X] grams of 2-(4 - iodophenyl)-3-(4 - nitrophenyl)-5-(2,4 - disulfophenyl)-2H - tetrazolium, monosodium salt. (Replace [X] with the actual quantity of the chemical in the packaging)
Storage	2-(4 - iodophenyl)-3-(4 - nitrophenyl)-5-(2,4 - disulfophenyl)-2H - tetrazolium, monosodium salt should be stored in a cool, dry place away from direct sunlight. Keep it in a tightly sealed container to prevent moisture absorption. Store it separately from incompatible substances. Ideal storage temperature is around 2 - 8 °C if possible, to maintain its stability and integrity.
Shipping	The chemical 2-(4 - iodophenyl)-3-(4 - nitrophenyl)-5-(2,4 - disulfophenyl)-2H - tetrazolium, monosodium salt is shipped in well - sealed containers. Special care is taken to prevent exposure, following strict regulations for chemical transport.

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2-(4-Iodophenyl)-3-(4-Nitrophenyl)-5-(2,4-Disulfophenyl)-2H-Tetrazolium,Monosodium Salt

General Information

Historical Development

The wonders of chemistry are deeply hidden. Today there is a thing named 2- (4 - Iodophenyl) -3- (4 - Nitrophenyl) -5- (2,4 - Disulfophenyl) -2H - Tetrazolium, Monosodium Salt. The first appearance of this thing originated from the intensive research of the sages.
Thinking back to the past, chemists worked hard and thought hard, and through the changes of time, after countless experiments, they came to this result. At the beginning, the understanding was still shallow, and only a few or two characteristics were briefly observed. However, the heart of a scholar is resolute and sensitive. With the passage of time, the research is deepened step by step, the analysis of its composition and structure is more and more detailed, and the reaction mechanism is also gradually getting a glimpse of the way. The journey experienced is like breaking through clouds and fog, and finally welcoming the dawn. So this thing has become famous from nowhere, occupying an important place in the chemical map, paving the way for subsequent research and enlightening new learning in the future.

Product Overview

##Product Overview
There is a product called 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazole, monosodium salt. This product is of great significance in the field of chemical research. Its unique structure is connected by iodophenyl, nitrophenyl, disulfonylphenyl and tetrazole structures, and forms a sodium salt.
This product is used in many biochemical experiments, or a key test drug. With its characteristics, it can participate in specific chemical reactions, helping researchers to understand the reaction mechanism and explore unknown laws. In the journey of experimental analysis, he often plays an indispensable role in injecting strength into scientific research progress, like a navigation beacon in the ocean of chemical mysteries, leading explorers to move forward and reveal more wonderful truths about the chemical world.

Physical & Chemical Properties

There is a thing named 2- (4 - Iodophenyl) -3- (4 - Nitrophenyl) -5- (2,4 - Disulfophenyl) -2H - Tetrazolium, Monosodium Salt. Looking at its physical properties, the shape is fixed, and the color quality is different. All kinds of physical characteristics are required for research.
As for the chemical properties, the combination of its components, the relationship between bond energy, and the trend of reaction all contain arcane. The molecular structure is exquisite, or in case of acid and base, temperature change, and light radiation, it can lead to different chemical changes. In case of suitable agents, there may be changes in decomposition, synthesis, and replacement, which are subtle and difficult to know without detailed investigation.
The study of the physical and chemical properties of this substance can be used in various fields of medicine, engineering, and scientific research, or it can be used skillfully, which can be the basis for development. Therefore, it is valuable to investigate its properties in detail.

Technical Specifications & Labeling

The study of chemistry is concerned with the properties and changes of substances. Today there is a thing named 2- (4 - Iodophenyl) -3- (4 - Nitrophenyl) -5- (2,4 - Disulfophenyl) -2H - Tetrazolium, Monosodium Salt. Its research also requires technical specifications and identification (product parameters).
Examine its chemical structure, and the groups cooperate to show a specific quality. The specification needs to specify its purity geometry, and the impurity is safe, which is related to the quality. The marker should be detailed about its physical and chemical properties, such as color state, melting point, boiling point, etc., and the user should know its properties and make good use of it. It is also necessary to show its preservation method to prevent quality change and maintain activity. In this way, it is of great benefit to those who study this object by meeting the technical specifications and identification methods.

Preparation Method

There is now a method for preparing 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazolium monosodium salt, as described in detail below. The raw materials used need to be selected from the best 4-iodoaniline, 4-nitrobenzaldehyde, etc. The synthesis process involves mixing 4-iodoaniline with related reagents in a delicate ratio, controlling the temperature to a suitable degree, and reacting when counted to obtain one of the intermediates. Then 4-nitrobenzaldehyde is reacted with another group of reagents according to regulations to form the second intermediate. Then the two intermediates meet, and under the action of a specific catalyst, after multiple steps of conversion, the reaction rate and temperature are carefully adjusted, which is the key. At the same time, various monitoring steps are set to prevent the growth of side reactions. After repeated purification and crystallization operations, the product is finally obtained, and each link is precisely controlled to achieve a good job.

Chemical Reactions & Modifications

In recent years, I have studied the chemical technology and found a reagent named "2- (4 - Iodophenyl) -3- (4 - Nitrophenyl) -5- (2,4 - Disulfophenyl) -2H - Tetrazolium, Monosodium Salt". The chemical reaction and modification of this agent, I deeply think it is important to study.
The chemical reaction of this agent is the transformation of multi-functional groups. The integration of iodobenzene, nitrobenzene and disulfonbenzene with tetrazolium monosodium salt, during which the migration of electron clouds and the change of bond energy can be followed. Iodine atoms have a strong absorption effect, which leads to charge bias in the reaction and promotes bond cracking and combination; the same is true for nitro groups, which adjust the reactivity due to their electron deficiency.
As for the way of modification, or the electronic properties of the substituent are adjusted to change the polarity of the molecule and the reaction check point; or the solvent conditions, the reaction rate and the product configuration are controlled. Such measures are expected to improve the performance of this agent and expand its application range. Only through careful research can it be used in the chemical industry to expand its new opportunities and create benefits.

Synonyms & Product Names

Today there is a thing named 2- (4-Iodophenyl) -3- (4-Nitrophenyl) -5- (2,4-Disulfophenyl) -2H-Tetrazolium, Monosodium Salt. This is a chemical substance, and it should have its alias and trade name in the industry.
However, it is crucial for me to look for its synonyms and trade names. Cover its different titles, or in various research and application scenarios, each has its own use. Scholars can accurately control this thing, analyze its nature, study its effect, and make it useful for scientific research and production. Or because of its structure and ability, it also has different names elsewhere. When people inquire about it, they know its equivalent, so that they can integrate it in relevant literature and materials, clarify the whole picture of this thing, and make it in the field of chemistry, contribute its energy, and create a new chapter in scientific research.

Safety & Operational Standards

Regarding the safety and operation specification of 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazolium monosodium salt
Fu2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazolium monosodium salt, this chemical product is related to safety and operation standards. It is of paramount importance and must not be ignored.
Store in a cool and dry place, away from water, fire and various heat sources. This is the basis for preventing its qualitative change. And it should be separated from oxidizing and reducing substances, and should not be mixed, so as not to cause harm.
As for the operation, the experimenter must wear complete protective gear. Bear the brunt, protective clothing is indispensable to protect the whole body. In addition, protective goggles should be worn to prevent them from splashing into the eyes and causing damage to the eyes. Protective gloves should also be worn to avoid skin contact.
In the operation room, it must be well ventilated, so that the foul gas can come out, and the clear air can enter, so as to prevent the experimenter from absorbing its harmful gas. Weigh this thing, use a precise device, and then gently move it to the desired place. Do not let it fall outside, if it is accidentally spilled, clean it up immediately. Wipe it with a damp cloth first, and then dispose of it properly.
During the reaction process, strictly follow the established process, control the temperature, duration, and dosage conditions, and do not make any mistakes. In case of emergencies, follow the emergency plan as soon as possible. After the experiment, the equipment used needs to be carefully cleaned, and the remnants should be properly disposed of, and should not be discarded at will.
In this way, strict adherence to safety and operating standards can ensure the smooth operation of the experiment, the safety of personnel, and the use of this chemical to the best of their ability, while avoiding all risks.

Application Area

There is a compound called 2- (4 - Iodophenyl) -3- (4 - Nitrophenyl) -5- (2,4 - Disulfophenyl) -2H - Tetrazolium, Monosodium Salt. This compound has unique functions in many application fields.
It can be used as a cell viability detection agent in the field of biochemical research. Through specific reactions, the relevant enzymes in the cell act on it, resulting in color changes. With this observation of the state of cell metabolism, it is clear that its vitality is of great help in medicine, drug research and development. Research assistants can understand cell behavior, explore the mechanism of diseases, and find drug targets. Furthermore, in some analytical testing fields, it can also rely on its characteristics to provide accurate and effective methods for component detection, substance quantification, etc., making complex sample analysis more smooth.

Research & Development

Recently used in chemistry, there is a research name 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazolium monosodium salt. This thing is quite important for experimental investigation. We have worked hard to study it to explore its structure, properties and reaction. After repeated tests, it has been proved that its activity in specific situations is to promote its progress and development in the field of reagents. It is hoped that through detailed investigation, this chemical can have outstanding performance in biochemical testing and medical research, blazing new trails, becoming a good tool, and contributing to the great cause of science, causing it to become increasingly prosperous, and making steady progress in the path of research and development.

Toxicity Research

Today there is a thing called 2- (4 - Iodophenyl) -3- (4 - Nitrophenyl) -5- (2,4 - Disulfophenyl) -2H - Tetrazolium, Monosodium Salt. As a chemical researcher, I am particularly concerned about the investigation of the toxicity of this thing.
Examine the properties of this thing carefully, and it is related to the safety of all things. The investigation of toxicity should not be ignored. When studying its toxicity, you can know its effect on life, whether it is harmful to humans and animals, or damaging to plants and trees.
When exploring, you should follow the scientific method and operate strictly. Observe its response to other things, and observe its changes in different environments. After many trials, the exact fruit can be obtained, and the strength and scope of its toxicity can be clearly understood. It is a warning to those who use this product, to ensure the safety of the four parties, and to prevent future disasters. Make this product used in the right way and avoid its disaster.

Future Prospects

The chemical substances that taste the world are all powerful tools for human beings and are of great use in various fields. Today there is a thing called "2- (4-Iodophenyl) -3- (4-Nitrophenyl) -5- (2,4-Disulfophenyl) -2H-Tetrazolium, Monosodium Salt", the potential of this material is unlimited.
Looking into the future, it can be used in the research of medical science, or it can help to explore the root cause of diseases, and it is a new path to cure diseases. In the field of biology, it may be able to understand the changes of living things and reveal the mysteries in the dark. Or it may be able to develop the industry of materials, creating strange materials, benefiting the people of the country and the people. The road ahead is not clear, but we should have great ambitions and devote ourselves to farming to explore its endless possibilities, so as to expand the New Territories of the future and live up to our expectations for the future.

Historical Development

2 - (4 - iodophenyl) -3 - (4 - nitrophenyl) -5 - (2,4 - disulfonic acid phenyl) -2H - tetrazolium monosodium salt. Although the development of this substance has not been heard of in ancient times, today's science has advanced, and this chemical has gradually appeared in the world. At the beginning, scholars devoted themselves to studying in the laboratory to explore its properties. After various attempts, analyzing its structure and measuring its reaction, its characteristics can be understood. From few to many, from sparse to dense, it has moved steadily on the road of scientific research. In the past, it has risen to prominence in various fields such as medicine and biochemistry. It is a weapon for scholars and helps the rise of scientific research. Just like a prairie fire, it is unstoppable. Its journey is a witness to scientific development, and it will also shine in the future.

Product Overview

Today, there is a product called 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazolium, a monosodium salt. This chemical product is commonly used in scientific research. Its unique properties can show specific effects in specific reactions. Looking at its structure, it is cleverly connected by several groups. Iodophenyl, nitrophenyl, disulfonylphenyl and tetrazolium ring are co-formed, and the state of sodium salt also affects its properties. In experiments, often due to its special structure, it can cause unique chemical changes, or be used for detection or catalysis, which adds a lot of help to chemical research and is an indispensable material in scientific research.

Physical & Chemical Properties

The so-called 2- (4-Iodophenyl) -3- (4-Nitrophenyl) -5- (2,4-Disulfophenyl) -2H-Tetrazolium, Monosodium Salt is related to its physical and chemical properties. The color state of this substance may be distinct, and the texture also has its own uniqueness. Regarding its solubility, whether it is soluble or not in a specific solvent depends on its chemical structure. Its physical constants such as melting point and boiling point are also its characteristics.
And its chemical activity varies under different reaction conditions. It can interact with various reagents to form new compounds. This is determined by the atomic bonding and electron distribution in its molecules. Therefore, the study of the physical and chemical properties of this substance can be used in various fields, such as medicine and chemical industry, to provide a base site and clarify its characteristics before it can be used effectively.

Technical Specifications & Labeling

Today there is a product named 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazolium, monosodium salt. In this product, process specifications and identification (product parameters) are the key. Looking at the process, it is necessary to follow a precise method, from raw material preparation to each step of reaction, all should be in line with the specifications, temperature control and speed regulation, and must not be sloppy at all. As for the logo, the ingredients, characteristics, uses, etc. should be detailed, so that those who see it know the details. This product parameter is related to the quality and quality, and it is also a criterion for measuring its usability. Those who make this product should be rigorous, adhere to the process specifications, and specify the details of the logo in order to obtain the best product.

Preparation Method

To prepare 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazolium monosodium salt, the method of preparation is related to the raw materials and production process, reaction steps and catalytic mechanism, which are of paramount importance.
Preliminary raw materials are based on carefully selected 4-iodoaniline, 4-nitrobenzaldehyde, 2,4-disulfonate phenylhydrazine, etc. In a suitable reaction vessel, add them in sequence to adjust the temperature, pressure and other conditions. The first step is to make 4-iodoaniline and 4-nitrobenzaldehyde condensation, which needs to be controlled at a certain temperature range and catalyzed by a specific catalyst. This step is related to the reaction rate and product purity.
Then add 2,4-disulfonate phenylhydrazine, and then adjust the reaction conditions to make it fully react. After the reaction is completed, the impurities are removed and the pure product is obtained after separation and purification. The whole process must be strictly controlled. According to the established process, high-quality 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H -tetraazolium monosodium salt can be prepared.

Chemical Reactions & Modifications

Fu2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazolium monosodium salt, its transformation and modification are the most important things for chemical researchers.
Under specific conditions, the reaction of this compound, various atoms and groups interact, or form a new structure. However, the reaction should be complex, subject to temperature, solvent, catalyst and other factors. If the temperature is not suitable, the reaction rate may be slow, and the product is impure; if the solvent is not suitable, or the reaction should be difficult.
As for the modification, it is designed to optimize its performance. Or by chemical modification, increase its stability, solubility, or change its reactivity. Modified, or can be extended in medicine, materials and other fields of application. If the chemical reaction can be well controlled, clever modification method, this compound will be able to develop its greater value, for the chemical contribution.

Synonyms & Product Names

There is a recent product called 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazolium, a monosodium salt. This product is quite important in our chemical research. It also has many synonymous names and commodity names.
The name of husband is synonymous, which is named from different perspectives in terms of its chemical structure and properties. The name of the product is related to the promotion and market circulation of the merchant.
If you explore this 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazole, the synonymous name of the monosodium salt and the name of the commodity, it is like seeking secrets. It can be consulted in detail in various classics and research documents. It is also necessary to look at its popular names in the chemical market, experimental applications, etc. After some investigation, you may get various synonymous names and commodity names, which will add a boost to the road of chemical research.

Safety & Operational Standards

2 - (4 - iodophenyl) - 3 - (4 - nitrophenyl) - 5 - (2,4 - disulfonic acid phenyl) - 2H - tetrazolium monosodium salt, this chemical substance is essential for safety and operation standards.
Its characteristics are different and have specific physical and chemical properties. When handling, the first priority is the suitability of the environment. When choosing a well-ventilated and well-lit place, avoid narrow occlusion to prevent the accumulation of harmful gases and endanger the health of the operator.
Furthermore, the operator must wear appropriate protective equipment. Wear goggles to prevent substances from splashing into the eyes and causing damage to the eyes; wear protective clothing to protect the contact between the skin and the substance; wear gloves to protect the safety of the hands.
When taking it, the method should be precise and careful. Measure with clean and suitable utensils, and avoid direct contact with the hands. After use, the utensils must be cleaned to avoid residue, which will cause mistakes in the next use.
There are also rules for storage. It should be stored in a cool, dry place, away from direct sunlight, and away from fire and heat sources, because it may be at risk of explosion. Different substances are stored separately according to their nature, and mixed storage is prohibited to prevent chemical reactions from causing danger.
If accidentally touched, quickly flush the contact area with a large amount of water. If it enters the eye, seek medical attention as soon as possible. If taken by mistake, do not induce vomiting, and seek medical help as soon as possible.
In short, the handling of 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonate phenyl) -2H-tetrazolium monosodium salt must strictly follow safety and operation regulations to ensure the safety of oneself and the environment.

Application Area

2 - (4 - iodophenyl) - 3 - (4 - nitrophenyl) - 5 - (2,4 - disulfonylphenyl) - 2H - tetrazolium monosodium salt is widely used in the field of biochemical pharmacology. It can be used for the detection of cell viability and proliferation. The dehydrogenase in the mitochondria of cells can reduce this salt to a colored product of methylbenzene, and the amount of the product is positively correlated with the number of living cells. Therefore, it has its shadow in many aspects such as drug screening and tumor research. During drug screening, it can be used to observe the response of cells to different drugs to judge the efficacy of drugs. In tumor research, it can help researchers understand the proliferation of tumor cells and provide a key basis for the development of anti-cancer drugs and the determination of treatment plans.

Research & Development

Taste the way of scientific research, the most important thing is to explore and develop. Today there is a thing named 2- (4 - Iodophenyl) -3- (4 - Nitrophenyl) -5- (2,4 - Disulfophenyl) -2H - Tetrazolium, Monosodium Salt, which is of great value in our scientific research.
The nature of this thing is related to the progress of many fields. Our generation should observe its changes and study its principles with a rigorous heart. Or we can explore its response through experiments and find its application. With time, if we can study it well, we will be able to make breakthroughs in the realm of scientific research and promote the development of this thing. It is hoped that it will add brilliance to the academic world, make the object shine in practical places, live up to the original intention of scientific research, and preserve usable results for future generations.

Toxicity Research

In recent years, Yu has devoted himself to the research of poisons, especially 2- (4 - Iodophenyl) -3- (4 - Nitrophenyl) -5- (2,4 - Disulfophenyl) -2H - Tetrazolium, Monosodium Salt. Its sexual toxicity is related to the safety of living beings, and research is urgent.
Observe this thing, want to understand its toxicity geometry. Or apply it to insects, observe the change of its behavior; or use it to plants, observe its state of prosperity and decline. After repeated tests, record the results in detail, hoping to know the little impact of its toxicity and the urgency of the attack.
Cover toxicity research, it is important for people's livelihood. Knowing its poison can prevent problems before they occur, ensure the well-being of all people, and protect the tranquility of all things. I will do my best to explore it, in the hope of obtaining it and using it for the world.

Future Prospects

Today there is a thing called 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonyphenyl) -2H-tetrazolium monosodium salt. As a chemical researcher, I look at this thing and think about its future prospects. This material is unique, or has extraordinary prospects in the field of biochemical detection. It can help accurately measure biological activity, gain insight into microscopic changes, and open up new paths for exploring the mysteries of life. It is also expected to make efforts in drug research and development, become a powerful tool for screening new drugs, accelerate the development of good drugs, and solve people's pain. Although there may be thorns in the road ahead, I firmly believe that with time and careful study, we will be able to tap its endless potential, bloom bright light, seek well-being for future generations, and open up unprecedented scenery.

Historical Development

I have tried to study the products of 2- (4 - Iodophenyl) -3- (4 - Nitrophenyl) -5- (2,4 - Disulfophenyl) -2H - Tetrazolium, Monosodium Salt. At the beginning, the sages did not know its nature, and it was difficult to explore. However, the public is diligent, and they are unremitting throughout the years. At the beginning, seek its nature at the end of the year, and observe its change in things. Then, with poor rationale, understand its rules in response to changes. As time goes by, the technology is new, and the research is more and more in-depth. I know that it can be used in medicine, can help in diagnosis and observation, and is of great use in the detection of micro-life and the judgment of disease. Therefore, its position in the world has gradually emerged, and it has been valued by various karma. It has not been revealed since the past. As for its wide use today, the development of this product depends on the efforts of various researchers and the work of time.

Product Overview

In October 2024, this chemical was named 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonate phenyl) -2H-tetrazolium monosodium salt. It is a special organic compound with a wide range of uses in the field of scientific research. The appearance is powdery, the color is often white or yellowish, and the texture is uniform and delicate.
This compound has a unique structure and contains key groups such as iodine, nitro, and sulfonic acid. The introduction of iodine atoms may cause it to have unique electronic effects and steric barriers, which affect the reactivity and intermolecular interactions. Nitro is a strong electron-absorbing group, which significantly changes the charge distribution and chemical properties of molecules. Sulfonic acid groups impart good water solubility and are beneficial for biological and medical research applications.
In scientific research, it is often used in biochemical experiments such as cell viability detection and enzyme activity analysis. In cytotoxicity studies, it can be used to react with specific enzymes in living cells to generate colored products, and cell viability can be judged according to the color depth, which helps to evaluate the impact of drugs and chemicals on cells.

Physical & Chemical Properties

There is now a product named 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonyphenyl) -2H-tetrazolium monosodium salt. Looking at its physical and chemical properties, this is the key to scientific research. Its chemical structure is unique, containing iodine, nitro and sulfonic acid groups and other groups. The introduction of iodine atoms may cause it to have unique reactivity and affect the intermolecular force. Nitro has strong electron absorption, which can change the electron cloud distribution of compounds, affecting their stability and reaction tendency. The disulfonyphenyl group makes the molecule have good water solubility and may have unique behavior in polar solvents. From the theory of physical properties, or due to the complex structure, the melting point and boiling point are special. Its color and shape are also determined by molecular structure. Only by studying this materialization property in detail can we open up new opportunities for related fields and pave the way for academic progress.

Technical Specifications & Labeling

Today, there is a product named 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonic acid phenyl) -2H-tetrazolium monosodium salt. In addition, the process specification and identification (product parameters) are the key.
Looking at the process specification, the selection of raw materials must be carefully selected, and the impurities must be small. The synthesis step must follow a rigorous process. The temperature, humidity and reaction time are fixed. A slight difference in the pool will affect the quality.
As for the identification, the product parameters should be clear and correct. The proportion of ingredients, physical properties, such as color, morphology, chemical properties, purity, etc. should be accurately marked. In this way, it can be trusted by users when it is applied, and it can play its due role in various fields without the risk of misuse.

Preparation Method

To prepare the product of 2 - (4 - iodophenyl) - 3 - (4 - nitrophenyl) - 5 - (2,4 - disulfonylphenyl) - 2H - tetrazolium monosodium salt, the method of preparing the product is essential. Prepare the materials first, 4 - iodoaniline, 4 - nitrophenylhydrazine, etc. are the raw materials, and each is taken according to the amount.
The combination step is to first couple 4 - iodoaniline with sodium nitrite in the acid solution to obtain a diazonium salt. Following the reaction of 4 - nitrophenylhydrazine with it, through the condensation step, it forms an intermediate product. Then take 2,4-disulfonylbenzaldehyde, co-place it with the intermediate, and at a suitable temperature and time, undergo a cyclization reaction to obtain a crude product.
After refining, it is dissolved in a solvent, filtered off impurities, and then crystallized and dried to obtain a pure product. This method of preparation requires rigorous temperature control, time control, and material control to obtain a high-quality product.

Chemical Reactions & Modifications

Reverse modification of the monosodium salts of furo2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazolium, as noted by our chemists. The reverse process often involves the combination of multi-element reversals.
The properties of this compound are characterized by iodine, nitro, and sulfonic acid groups. In the reaction, the iodine group or the nucleus can be substituted, and the nitro group can be separated into clouds, causing the reaction activity to be changed. The sulfonic acid group also affects the reaction rate due to its resistance.
Modified, or new groups can be introduced to improve its physical and chemical properties. Or change its solubility, or increase its qualitative value, in order to expand its application in multiple domains. All of this requires us to study the reverse theory in order to achieve fine control and hope to make this compound more effective.

Synonyms & Product Names

Today, there is a product named 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazolium monosodium salt. This product is widely used in the field of chemistry. It also has many synonymous names and trade names.
All synonymous names refer to this product accurately to conform to the norms of chemical expression. And the trade name is the specific name taken by the merchant to facilitate marketing activities and distinguish the same kind. In the process of scientific research, it is extremely crucial to know the synonymous name and trade name of this product. Synonymous names help researchers communicate and exchange results accurately, avoiding understanding biases due to differences in terms; trade names enable researchers to understand various products in the market, so they can choose suitable items and benefit scientific research progress. These are all indispensable elements in chemical research and play an important role in the search for scientific truth.

Safety & Operational Standards

2 - (4 - iodophenyl) - 3 - (4 - nitrophenyl) - 5 - (2,4 - disulfonic acid phenyl) - 2H - tetrazolium monosodium salt, this chemical substance is related to safety and operating standards, and is very important.
This chemical substance has different properties, and special attention should be paid to safety. If you hide it, you must choose a cool and dry place, away from water and fire, and away from all kinds of heat sources. Cover it or be very sensitive to temperature and humidity. If you are not careful, you may be afraid of changes. And do not coexist in a room with various strong oxidizing and reducing agents to prevent sudden reactions and endanger the safety of the surrounding area.
As for the operation, be sure to follow strict procedures. The operator must wear protective gear. If it is protective clothing, goggles, or gloves, do not let the skin or eyes come into contact with it. When weighing, it is advisable to use a precise device. Do it according to the law, and do not be hasty. The steps of dissolving and mixing must also be slow and orderly. Check the reaction status. If there is any abnormality, stop it quickly.
After the reaction is completed, the disposal of its waste should not be ignored. According to environmental protection regulations, classify and place it in a special disposal agency. Do not discard it in the wild at will, so as not to pollute the environment and harm life.
In short, the safety and operation of 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonate phenyl) -2H-tetrazolium monosodium salt should be handled with awe and in accordance with the regulations, so as to ensure that everything goes smoothly and is harmless.

Application Area

Today, there is a product called 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazolium monosodium salt. This product has a wide range of uses in the field of biochemistry.
It can be used as a key reagent in the detection of cell viability. When cell metabolism is active, this product will be reduced by enzymes in the cell to produce a colored product. By measuring the color depth of this product, it can be known that the level of cell viability is high and low, and it has an important guiding effect in many aspects such as pharmaceutical research and development, tumor research, etc.
It can also play a role in the context of immunoassay. With its characteristics, it can assist in the detection of specific antigens or antibodies, providing accurate assistance for disease diagnosis, pathogen detection, etc., and contributing to the progress of medical science.

Research & Development

There are chemical substances in modern times, named 2- (4 - Iodophenyl) -3- (4 - Nitrophenyl) -5- (2,4 - Disulfophenyl) -2H - Tetrazolium, Monosodium Salt, I will devote myself to research. This substance is quite important in the process of research and use.
I have worked hard all day and night to explore its nature and observe its transformation under various conditions. Observe its response to other things, and analyze the wonders of its structure. At the beginning, I encountered many difficulties, but I did not dare to slack off.
After months of study, its characteristics and reaction mechanism have gradually come to an understanding. In the field of biochemistry, it may be of great use, which can help the accuracy of testing and promote the progress of scientific research. I hope to use this as a basis, explore it in depth, and hope to create something, so as to promote the wide use of this substance and contribute to the development of chemistry.

Toxicity Research

Recently, I have been focusing on a thing named 2- (4 - Iodophenyl) -3- (4 - Nitrophenyl) -5- (2,4 - Disulfophenyl) -2H - Tetrazolium, Monosodium Salt. This thing is related to toxicity research and is quite crucial.
I look at ancient books. The test of toxicity is to observe its impact on living beings. Or put it in water and observe the state of the aquarium; or apply it to soil and observe the change of grass and trees.
The toxicity of this thing in the present study is also based on ancient principles. First apply it to small things, such as insects and mice, in microdoses. Observe the changes in its diet, action, and reproduction. If the diet is gradually reduced, the movement is slow, and the reproduction is damaged, the toxicity will be obvious. Also observe the reaction of its entry into the body, whether there are any abnormal conditions, such as boils on the body, or internal incompatibility.
The study of toxicity is not a day's work. Long-term observation and careful recording are required, and then the depth of its toxicity can be determined. Those who use this product in future generations should be well-prepared to avoid its harm and pursue its benefits.

Future Prospects

In today's world, technology is changing with each passing day, and chemical things are also evolving. Today there is a thing called "2- (4-Iodophenyl) -3- (4-Nitrophenyl) -5- (2,4-Disulfophenyl) -2H-Tetrazolium, Monosodium Salt".
As chemical researchers, we often think about the future prospects of this thing. Its use in scientific research may open up new avenues. In the field of biochemistry, it can assist in the study of cell changes, enzyme activities, and explore the mystery of life.
With time, or with its characteristics, an efficient detection method can be created, which will open the door to convenience for the pharmaceutical, environmental protection and other industries. It is also expected to improve its properties, making it more widely used and more effective. Although the road ahead is long, we hold the heart of research and look forward to the future, so that this object can exhibit extraordinary capabilities, be used by the world, and promote human progress.

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Frequently Asked Questions

As a leading 2-(4-Iodophenyl)-3-(4-Nitrophenyl)-5-(2,4-Disulfophenyl)-2H-Tetrazolium,Monosodium Salt supplier, we deliver high-quality products across diverse grades to meet evolving needs, empowering global customers with safe, efficient, and compliant chemical solutions.

What is the chemical structure of 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfophenyl) -2h-tetrazolium, monosodium Salt?

2-% 284-iodophenyl% 29-3-% 284-nitrophenyl% 29-5-% 282% 2C4-disulfonylphenyl% 29-2H-tetrazolium monosodium salt, its chemical structure is more complex. According to its name, this is an organic compound containing various functional groups such as iodine, nitro, and sulfonyl.
This substance is composed of a tetrazolium ring as the core skeleton. It is connected to 4-iodophenyl at the 2-position, and the iodine atom is attached to the 4-position of the benzene ring. The 3-position is connected to 4-nitrophenyl, and the nitro group is also in the 4-position of the benzene ring. The 5-position is connected to 2,4-disulfonylphenyl, that is, the 2-position and 4-position of In addition, due to the monosodium salt, it can be seen that there is a hydrogen atom on the tetrazolium ring replaced by sodium ions to form a salt.
Its structure design is exquisite, and each group interacts, giving this compound unique chemical and physical properties. Different functional groups affect its solubility and reactivity, etc. It may have specific uses in chemical, biological and other research fields, or as a biochemical reaction reagent, or for specific detection, depending on its structural characteristics.

What are the main uses of 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfophenyl) -2h-tetrazolium, monosodium Salt?

2-% 284-iodophenyl% 29-3-% 284-nitrophenyl% 29-5-% 282% 2C4-disulfonic acid phenyl% 29-2H-tetrazolium monosodium salt, often referred to as its English abbreviation INT, has important uses in biochemistry, medicine, agriculture and other fields.
In the field of cell viability and proliferation detection, INT has an extraordinary effect. Cell viability is related to the ability of cells to maintain normal physiological functions, and proliferation refers to the process of increasing the number of cells due to division. Under specific experimental conditions, living cells have the ability to reduce INT. After this reduction, INT is transformed from its original color state to a product of methyl. The color of this product is bright, and its absorbance can be measured by specific instruments. Through accurate measurement and analysis of absorbance, researchers can gain a keen insight into cell viability and proliferation. For example, in the experiment of anti-cancer drug development, treating cancer cells with different concentrations of anti-cancer drugs, and then detecting the amount of products produced by INT, can clarify the effect of drugs on the viability and proliferation of cancer cells, and provide key data support for evaluating drug efficacy.
In the field of microbial detection, INT also plays a significant role. Microorganisms exist widely in the natural environment and many production activities, and the monitoring of their types and quantities is of great significance. Some microorganisms can reduce INT during metabolism to form a colored nail. Qualitative and quantitative analysis of specific microorganisms can be realized by observing whether there is nail formation and the amount of nail. For example, in water quality testing, if there are microorganisms that can reduce INT in the water, it will generate a nail, and the color of the nail can be determined according to the depth of the nail, which can preliminarily judge the degree of microbial pollution in the water and provide an effective basis for water quality safety.
In plant physiology research, INT is also indispensable. Plant cells can also reduce INT under specific physiological states. Researchers can use INT testing to explore the changes in cell viability of plants under different environmental stresses (such as drought, high temperature, heavy metal pollution, etc.). For example, when plants are under drought stress, using INT to detect the cell viability of plant leaves can provide a theoretical basis for the cultivation of drought-tolerant plant varieties.

What are the physical properties of 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfophenyl) -2h-tetrazolium, monosodium Salt?

2-% 284-Iodophenyl% 29-3-% 284-nitrophenyl% 29-5-% 282% 2C4-disulfonic acid phenyl% 29-2H-tetrazolium monosodium salt, this is the name of the chemical substance. Its physical properties are quite important, related to its many uses and characteristics.
This substance is usually in solid form. In appearance, it often takes the form of a powder with a specific color. The specific color may vary depending on the purity and preparation method. It is usually a slightly colored powder, which is not colorless and pure.
In terms of melting point, due to the complex molecular structure, it contains iodine, nitro, sulfonic acid groups and other functional groups. The interaction between these groups results in a specific melting point range. However, the exact melting point needs to be determined by professional experimental instruments, and different literatures may vary slightly due to differences in experimental conditions.
Solubility is also a key physical property. Because of its sulfonic acid group, this group is hydrophilic, so the substance has a certain solubility in water, which can be partially dissolved to form a uniform dispersion system. However, the solubility is also affected by factors such as temperature. In organic solvents, the solubility varies according to the polarity of the solvent, and the solubility in polar organic solvents may be higher than that in non-polar solvents.
In terms of stability, due to the nitro group and iodine atoms in the structure, nitro is oxidizing, and iodine atoms are relatively active, so under specific conditions, such as high temperature, light or contact with specific chemicals, chemical reactions may occur, and the stability is limited.
In summary, the physical properties of 2-% 284-iodophenyl% 29-3-% 284-nitrophenyl% 29-5-% 282% 2C4-disulfonic acid phenyl% 29-2H-tetrazolium monosodium salt, such as appearance, melting point, solubility and stability, are determined by its unique molecular structure and have a profound impact on its application in various fields.

2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfophenyl) -2h-tetrazolium, monosodium Salt What are the precautions during use?

2 - (4 - iodophenyl) - 3 - (4 - nitrophenyl) - 5 - (2,4 - disulfonic acid phenyl) - 2H - tetrazolium monosodium salt, commonly known as tetrazolium blue (MTT), is also used in experimental reagents. When using, pay attention to the following things:
First, this agent is very sensitive to light, it should be stored away from light, and it should be used as much as possible to avoid strong light, so as not to lose its properties due to photolysis, resulting in inaccurate experimental results. If exposed to light for too long, its structure or change, activity will decline, and the measured data will also lose its authenticity.
Second, the dissolution of MTT is quite critical. Commonly used organic solvents such as dimethyl sulfoxide (DMSO) are used to dissolve it. When dissolving, it must be stirred evenly and ensure complete dissolution. If there are undissolved particles entering the reaction system, it will interfere with the experiment and make the results messy.
Third, the pH value of the experimental system should not be ignored. The activity of this agent is stable within a specific pH range, so it is crucial to adjust the pH of the system. pH discomfort, or abnormal reaction between MTT and the test object, resulting in experimental deviation.
Fourth, during use, the amount of MTT added should be strictly controlled. Too much or too little addition will affect the experimental results. Add too much, or cause the signal to be too strong, beyond the detection range; add too little, the signal is weak, it is difficult to accurately measure.
Fifth, this agent has a certain toxicity, when operating, beware. Do not let it come into contact with the skin, eyes, etc. If you accidentally touch it, rinse it with a lot of water quickly, and seek medical attention if necessary. And after the experiment, waste containing MTT should be properly disposed of according to regulations, do not discard it at will, so as not to pollute the environment.

What is the market price of 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfophenyl) -2h-tetrazolium, monosodium Salt?

It is asked today that the price of 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazolium monosodium salt is about what the market price is. This is a fine chemical reagent, and its price often varies depending on quality, source, and purchase quantity.
If a small amount is purchased from a common chemical reagent, it may be expensive to meet the needs of the experiment. The preparation process of this reagent may be complex, and the raw materials may be rare. If the quality and purity are high, the price per gram may reach several hundred yuan.
However, if it is for industrial use, the purchase volume is quite large, and the price can be reduced with the manufacturer. If the quantity is in kilograms, the price per gram may drop to tens of yuan due to the benefit of scale.
Market fluctuations and changes in supply and demand also affect its price. When demand is greater than supply, the price may rise; if supply exceeds demand, the price may be depressed. To know the exact price, when consulting the reagent supplier and comparing its quotation, a more accurate number can be obtained.