What is the chemical structure of 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfophenyl) -2h-tetrazolium, monosodium Salt?

2-% 284-iodophenyl% 29-3-% 284-nitrophenyl% 29-5-% 282% 2C4-disulfonylphenyl% 29-2H-tetrazolium monosodium salt, its chemical structure is more complex. According to its name, this is an organic compound containing various functional groups such as iodine, nitro, and sulfonyl.

This substance is composed of a tetrazolium ring as the core skeleton. It is connected to 4-iodophenyl at the 2-position, and the iodine atom is attached to the 4-position of the benzene ring. The 3-position is connected to 4-nitrophenyl, and the nitro group is also in the 4-position of the benzene ring. The 5-position is connected to 2,4-disulfonylphenyl, that is, the 2-position and 4-position of In addition, due to the monosodium salt, it can be seen that there is a hydrogen atom on the tetrazolium ring replaced by sodium ions to form a salt.

Its structure design is exquisite, and each group interacts, giving this compound unique chemical and physical properties. Different functional groups affect its solubility and reactivity, etc. It may have specific uses in chemical, biological and other research fields, or as a biochemical reaction reagent, or for specific detection, depending on its structural characteristics.

What are the main uses of 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfophenyl) -2h-tetrazolium, monosodium Salt?

2-% 284-iodophenyl% 29-3-% 284-nitrophenyl% 29-5-% 282% 2C4-disulfonic acid phenyl% 29-2H-tetrazolium monosodium salt, often referred to as its English abbreviation INT, has important uses in biochemistry, medicine, agriculture and other fields.

In the field of cell viability and proliferation detection, INT has an extraordinary effect. Cell viability is related to the ability of cells to maintain normal physiological functions, and proliferation refers to the process of increasing the number of cells due to division. Under specific experimental conditions, living cells have the ability to reduce INT. After this reduction, INT is transformed from its original color state to a product of methyl. The color of this product is bright, and its absorbance can be measured by specific instruments. Through accurate measurement and analysis of absorbance, researchers can gain a keen insight into cell viability and proliferation. For example, in the experiment of anti-cancer drug development, treating cancer cells with different concentrations of anti-cancer drugs, and then detecting the amount of products produced by INT, can clarify the effect of drugs on the viability and proliferation of cancer cells, and provide key data support for evaluating drug efficacy.

In the field of microbial detection, INT also plays a significant role. Microorganisms exist widely in the natural environment and many production activities, and the monitoring of their types and quantities is of great significance. Some microorganisms can reduce INT during metabolism to form a colored nail. Qualitative and quantitative analysis of specific microorganisms can be realized by observing whether there is nail formation and the amount of nail. For example, in water quality testing, if there are microorganisms that can reduce INT in the water, it will generate a nail, and the color of the nail can be determined according to the depth of the nail, which can preliminarily judge the degree of microbial pollution in the water and provide an effective basis for water quality safety.

In plant physiology research, INT is also indispensable. Plant cells can also reduce INT under specific physiological states. Researchers can use INT testing to explore the changes in cell viability of plants under different environmental stresses (such as drought, high temperature, heavy metal pollution, etc.). For example, when plants are under drought stress, using INT to detect the cell viability of plant leaves can provide a theoretical basis for the cultivation of drought-tolerant plant varieties.

What are the physical properties of 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfophenyl) -2h-tetrazolium, monosodium Salt?

2-% 284-Iodophenyl% 29-3-% 284-nitrophenyl% 29-5-% 282% 2C4-disulfonic acid phenyl% 29-2H-tetrazolium monosodium salt, this is the name of the chemical substance. Its physical properties are quite important, related to its many uses and characteristics.

This substance is usually in solid form. In appearance, it often takes the form of a powder with a specific color. The specific color may vary depending on the purity and preparation method. It is usually a slightly colored powder, which is not colorless and pure.

In terms of melting point, due to the complex molecular structure, it contains iodine, nitro, sulfonic acid groups and other functional groups. The interaction between these groups results in a specific melting point range. However, the exact melting point needs to be determined by professional experimental instruments, and different literatures may vary slightly due to differences in experimental conditions.

Solubility is also a key physical property. Because of its sulfonic acid group, this group is hydrophilic, so the substance has a certain solubility in water, which can be partially dissolved to form a uniform dispersion system. However, the solubility is also affected by factors such as temperature. In organic solvents, the solubility varies according to the polarity of the solvent, and the solubility in polar organic solvents may be higher than that in non-polar solvents.

In terms of stability, due to the nitro group and iodine atoms in the structure, nitro is oxidizing, and iodine atoms are relatively active, so under specific conditions, such as high temperature, light or contact with specific chemicals, chemical reactions may occur, and the stability is limited.

In summary, the physical properties of 2-% 284-iodophenyl% 29-3-% 284-nitrophenyl% 29-5-% 282% 2C4-disulfonic acid phenyl% 29-2H-tetrazolium monosodium salt, such as appearance, melting point, solubility and stability, are determined by its unique molecular structure and have a profound impact on its application in various fields.

2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfophenyl) -2h-tetrazolium, monosodium Salt What are the precautions during use?

2 - (4 - iodophenyl) - 3 - (4 - nitrophenyl) - 5 - (2,4 - disulfonic acid phenyl) - 2H - tetrazolium monosodium salt, commonly known as tetrazolium blue (MTT), is also used in experimental reagents. When using, pay attention to the following things:
First, this agent is very sensitive to light, it should be stored away from light, and it should be used as much as possible to avoid strong light, so as not to lose its properties due to photolysis, resulting in inaccurate experimental results. If exposed to light for too long, its structure or change, activity will decline, and the measured data will also lose its authenticity.
Second, the dissolution of MTT is quite critical. Commonly used organic solvents such as dimethyl sulfoxide (DMSO) are used to dissolve it. When dissolving, it must be stirred evenly and ensure complete dissolution. If there are undissolved particles entering the reaction system, it will interfere with the experiment and make the results messy.
Third, the pH value of the experimental system should not be ignored. The activity of this agent is stable within a specific pH range, so it is crucial to adjust the pH of the system. pH discomfort, or abnormal reaction between MTT and the test object, resulting in experimental deviation.
Fourth, during use, the amount of MTT added should be strictly controlled. Too much or too little addition will affect the experimental results. Add too much, or cause the signal to be too strong, beyond the detection range; add too little, the signal is weak, it is difficult to accurately measure.
Fifth, this agent has a certain toxicity, when operating, beware. Do not let it come into contact with the skin, eyes, etc. If you accidentally touch it, rinse it with a lot of water quickly, and seek medical attention if necessary. And after the experiment, waste containing MTT should be properly disposed of according to regulations, do not discard it at will, so as not to pollute the environment.

What is the market price of 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfophenyl) -2h-tetrazolium, monosodium Salt?

It is asked today that the price of 2- (4-iodophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfonylphenyl) -2H-tetrazolium monosodium salt is about what the market price is. This is a fine chemical reagent, and its price often varies depending on quality, source, and purchase quantity.

If a small amount is purchased from a common chemical reagent, it may be expensive to meet the needs of the experiment. The preparation process of this reagent may be complex, and the raw materials may be rare. If the quality and purity are high, the price per gram may reach several hundred yuan.

However, if it is for industrial use, the purchase volume is quite large, and the price can be reduced with the manufacturer. If the quantity is in kilograms, the price per gram may drop to tens of yuan due to the benefit of scale.

Market fluctuations and changes in supply and demand also affect its price. When demand is greater than supply, the price may rise; if supply exceeds demand, the price may be depressed. To know the exact price, when consulting the reagent supplier and comparing its quotation, a more accurate number can be obtained.