2 4 Iodophenyl 3 4 Nitrophenyl 5 Phenyltetrazolium Chloride Hydrate

The chemical structure of 2-% 284-iodophenyl% 29-3-% 284-nitrophenyl% 29-5-phenyl tetrazolium chloride hydrate is as follows.
The core of this compound is a tetrazolium ring, which is a five-membered heterocycle composed of five atoms, four of which are nitrogen atoms and one is carbon atoms. On this basic structure, there is a 4-iodophenyl group connected at the 2-position. The so-called 4-iodophenyl group is a group formed by the substitution of the 4-position of the benzene ring (opposite the bond connecting the tetrazolium ring) by an iodine atom. The benzene ring is a conjugated ring structure composed of six carbon atoms, which has a unique electron cloud distribution and chemical activity. The
3-position is connected with 4-nitrophenyl group. 4-nitrophenyl group, that is, the 4-position of the benzene ring is replaced by nitro (-NO 2). Nitro has strong electron-absorbing properties, which has a great impact on the electron cloud distribution and chemical properties of the whole molecule. The phenyl group connected to the
5-position is a simple benzene ring structure. The electron cloud conjugation system of the benzene ring makes it able to participate in a variety of electronic effects and chemical reactions.
Furthermore, this compound contains chloride ions (Cl) to balance the charge of the entire molecule and form a stable salt structure. At the same time, it is a hydrate, which means that a certain proportion of water molecules are bound in the compound structure, and there are hydrogen bonds between the water molecules and the main compound, which affect the physical properties of the compound, such as solubility and crystal structure. Such a chemical structure endows the compound with unique physical and chemical properties, which may have specific applications in many chemical and biological fields.

2-% 284-iodophenyl% 29-3-% 284-nitrophenyl% 29-5-phenyltetrazolium chloride hydrate, which is 2- (4-iodophenyl) -3- (4-nitrophenyl) -5 -phenyltetrazolium Chloride Hydrate, often referred to as INT chloride hydrate. This substance has a wide range of uses and is of great significance in the field of biochemical research.
First, it plays a significant role in the detection of cell viability and proliferation. Dehydrogenase in the mitochondria of cells can reduce INT chloride hydrate to insoluble methanide products, and the amount of production is proportional to the number of living cells. Therefore, by colorimetric determination of the absorbance of methanide products, cell viability and proliferation can be accurately evaluated. This detection method is widely used in drug screening, cytotoxicity testing, and tumor cell growth research. For example, in drug development, this test can be used to quickly determine the impact of drugs on cell viability and screen potential drug candidates.
Second, it also has important uses in the field of microbial detection. For microorganisms such as bacteria and fungi, their metabolic activities can promote the reduction reaction of INT chloride hydrates. According to the degree of reduction, the growth status and metabolic activity of microorganisms can be understood. In environmental microbial detection, it can be used to evaluate the activity and quantity of microorganisms in environmental samples such as water and soil, and help to judge environmental quality and ecological status.
Third, in plant physiology research, INT chloride hydrate can also be used to detect the viability and metabolic activity of plant cells. When plants suffer from stress, cell viability will change. Using INT chloride hydrate detection can explore the physiological response mechanism of plants under stress conditions such as drought, high temperature and low temperature, and provide theoretical basis for improving plant stress resistance.

2-% 284-iodophenyl% 29-3-% 284-nitrophenyl% 29-5-phenyltetrazolium chloride hydrate is a commonly used reagent in chemical research. This material has unique physical properties and is worth exploring.
Looking at its morphology, it is mostly crystalline at room temperature, with a yellow or orange appearance, a regular crystal structure, and a delicate surface luster. It is carefully carved like a miniature gem, which shines with a unique light under light.
Talking about solubility, this compound exhibits good solubility in many organic solvents. For example, in common ethanol and dimethyl sulfoxide, it can be miscible in a certain proportion. The polarity of ethanol interacts with some groups of the compound, so that the molecules can be uniformly dispersed to form a uniform and stable solution system. In dimethyl sulfoxide, its strong polar environment is more conducive to the dissolution of the substance, providing an excellent medium for related reactions.
Furthermore, the melting point is an important parameter to characterize the characteristics of the substance. Its melting point is relatively high, which is due to the strong interaction force between molecules. The iodine atom, nitro, phenyl and other groups in the molecule are closely connected through van der Waals force, hydrogen bonding, etc. It takes a higher temperature to destroy this structure, causing the substance to change from solid to liquid, providing an important reference for experimental operation and reaction conditions setting.
In addition, its density is also a property that cannot be ignored. Appropriate density data is of great significance for experimental operations such as accurate measurement and calculation of the proportion of the reaction system, ensuring the accuracy and reproducibility of the experimental results.
In summary, the morphology, solubility, melting point, and density of 2-% 284-iodophenyl% 29-3-% 284-nitrophenyl% 29-5-phenyl tetraazole chloride hydrate are related to each other, which together constitute its unique physical properties and lay a solid foundation for chemical research and related applications.

2-% 284-iodophenyl% 29-3-% 284-nitrophenyl% 29-5-phenyltetrazolium chloride hydrate. The method of synthesizing this substance is an important matter in chemistry. The method is also usually started by aryl nitriles. First take 4-iodobenzonitrile, 4-nitrobenzonitrile and benzonitrile in an appropriate amount, and place it in the reactor. Add an appropriate amount of solvent, such as dimethylformamide, to facilitate uniform reaction. Then add sodium azide, which is a key reagent, and it can cyclize with nitriles. The temperature needs to be controlled in a suitable range, about 60 to 80 degrees Celsius, to prevent side reactions from happening. During the reaction, keep stirring to ensure that the substances are fully mixed. After the reaction is completed, the product is separated by an appropriate method, or by column chromatography, using silica gel as the filler, a suitable eluent, such as a mixture of petroleum ether and ethyl acetate, is selected, and the product is separated from the reaction mixture. After recrystallization, ethanol or acetone is used as the solvent to purify the product, and a pure 2-% 284-iodophenyl% 29-3-% 284-nitrophenyl% 29-5-phenyl tetrazolium chloride hydrate can be obtained. In this process, the proportion of various substances, the temperature and time of the reaction need to be carefully adjusted to obtain satisfactory yield and purity.

2-% 284-Iodophenyl% 29-3-% 284-nitrophenyl% 29-5-phenyltetrazolium chloride hydrate, when using, there are many things to pay attention to. This reagent has specific chemical properties and needs to be treated with caution.
First, it is related to the preservation method. Because it may be quite sensitive to environmental factors, it should be stored in a dry, cool and well-ventilated place. It is appropriate to avoid light and shade in a dark place to prevent light, humidity and high temperature from causing deterioration and affecting the use effect.
Second, when taking it, be sure to operate it carefully. Because it is a chemical substance, or has certain toxicity and irritation, it is necessary to strictly follow the experimental specifications, wear appropriate protective equipment, such as gloves, goggles, etc., to prevent contact with the skin and eyes, and to avoid inhaling its dust.
Furthermore, during use, it is crucial to control the reaction conditions. The chemical reactions it participates in, temperature, pH, reaction time and other factors may have a profound impact on the reaction process and product generation. Therefore, before the experiment, it is necessary to plan carefully and precisely control the conditions to ensure the accuracy and reliability of the experimental results.
In addition, after use, the disposal of the remaining reagents should not be underestimated. Do not discard at will, and should be properly disposed of in accordance with the chemical waste treatment regulations to prevent environmental pollution.
In conclusion, the use of 2-% 284-iodophenyl% 29-3-% 284-nitrophenyl% 29-5-phenyltetrazolium chloride hydrate requires rigorous operation from start to finish, paying attention to all details, in order to ensure the safety and smooth progress of the experiment.