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What is the chemical structure of 3- (p-iodophenyl) -2- (p-nitrophenyl) -5-phenyl Tetrazolium Chloride?
3 - (p-iodophenyl) - 2 - (p-nitrophenyl) - 5 - phenyl tetrazolium chloride, this is an organic compound. Looking at its name, its chemical structure can be roughly deduced. "3- (p-iodophenyl) " indicates that at the 3rd position of the tetrazolium chloride ring, there is a pair of -iodophenyl groups connected. On this pair of -iodophenyl, that is, the phenyl ring, the iodine atom is located in the opposite position to the bond connecting the tetrazolium ring. " 2 - (p-nitrophenyl) ", which means that there is a pair of -nitrophenyl groups connected to the second position of the tetrazole ring chloride, that is, on the benzene ring, the nitro group is in the opposite position to the bond connecting the tetrazole ring. And" 5-phenyl "is connected to a phenyl group at the fifth position of the tetrazole ring chloride. The tetrazole ring itself consists of four nitrogen atoms and one carbon atom to form a five-membered ring structure with a chlorine atom. In summary, the chemical structure of this compound is composed of a tetrazole ring chloride as the core, and is connected to p-nitrophenyl, p-iodophenyl and phenyl at its 2,3,5 positions, respectively.
What are the main uses of 3- (p-iodophenyl) -2- (p-nitrophenyl) -5-phenyl Tetrazolium Chloride?
3 - (p-iodophenyl) - 2 - (p-nitrophenyl) - 5 - phenyl tetrazole chloride, which has a wide range of uses.
In the field of biochemical research, it is often used as a reagent for activity detection such as dehydrogenase. The substance can react under the action of specific enzymes to generate colored products. By detecting the color change, the activity of the enzyme can be known. For example, in cell respiration-related studies, intracellular dehydrogenase prompts it to react, and the color depth is used to measure the respiration rate of cells, which helps researchers understand the activity of cell metabolism.
In the process of drug development, it also has an important function. It can be used to evaluate the effect of drugs on cell metabolism. Taking the research and development of anti-cancer drugs as an example, drugs are applied to cancer cells to detect metabolic changes in cancer cells, and to observe the inhibitory effect of drugs on cancer cell growth, providing a key basis for drug screening and efficacy evaluation.
In terms of microbial detection, different microbial species can be distinguished. Different microorganisms respond differently to them due to different metabolic characteristics. With this characteristic, microbial categories in environmental samples or clinical samples can be identified, which is of great significance for disease diagnosis and environmental microbial research.
In the field of agriculture, plant seed vitality can be detected. Seeds with high vitality have strong metabolism and obvious reaction with this substance. According to the degree of reaction, the seed vitality can be judged, providing assistance for seed quality control and seed selection and breeding.
How to use 3- (p-iodophenyl) -2- (p-nitrophenyl) -5-phenyl Tetrazolium Chloride?
3 - (p-iodophenyl) - 2 - (p-nitrophenyl) - 5 - phenyl tetrazole chloride, often referred to as INT Chloride in English. Its usage is as follows:
This substance is mostly used in biochemical and medical experiments to detect specific reactions in living organisms. In cell viability and proliferation detection experiments, its use is quite similar to the ancient method of probing. When the cell is active, the dehydrogenase in the mitochondria can cause the reduction reaction of INT Chloride. This reduction process is like the ancient alchemy, which converts the colorless INT Chloride into the red nail product. By measuring the absorbance of the product at a specific wavelength, it is possible to accurately infer the cell viability and proliferation status like a celestial phenomenon.
In immunohistochemical staining experiments, INT Chloride is also useful. It is like an ancient marker, which can bind to a specific antibody, and then mark the location of the target antigen. For specific operation, the tissue section is incubated with the primary antibody first. This process is like searching for a soulmate, and the primary antibody accurately recognizes the target antigen. Then an enzyme-labeled secondary antibody conjugated to INT Chloride is added, which acts as a messenger and brings INT Chloride to the target area. Under the catalysis of the enzyme, INT Chloride reacts and generates a colored precipitate, so that the target antigen can be clearly seen in the field of vision, just like a monument.
When using, be sure to follow the operating specifications carefully. First of all, the dissolution of INT Chloride requires a suitable solvent, such as dimethyl sulfoxide (DMSO). The dissolution process should be like toning and stirring slowly to promote uniform dispersion. Secondly, the concentration used in the experiment should be accurately proportioned according to the specific experimental requirements, such as the ancient method of processing medicinal pills. Furthermore, the reaction conditions also need to be strictly controlled. Temperature and time are the key. If the temperature is controlled, a slight difference will affect the accuracy of the experimental results. Only in this way can we borrow the power of INT Chloride to obtain accurate and reliable experimental data.
What should I pay attention to when storing 3- (p-iodophenyl) -2- (p-nitrophenyl) -5-phenyl Tetrazolium Chloride?
3 - (p-iodophenyl) - 2 - (p-nitrophenyl) - 5 - phenyl tetrazolium chloride, this is a chemical reagent. When storing, many things need to be paid attention to.
First, pay attention to the storage temperature. This reagent should be stored in a low temperature environment to slow down its chemical reaction rate and ensure the stability of chemical properties. Generally speaking, it is best to maintain the temperature at 2-8 ° C. It is like being placed in a refrigerated environment, which can effectively avoid decomposition or deterioration due to excessive temperature.
Second, the control of humidity is also crucial. It should be stored in a dry place away from moisture. Because moisture can easily cause deliquescence of the reagent, which in turn affects its purity and performance. It can be placed in a desiccant dryer to ensure a dry environment.
Third, pay attention to the influence of light. The reagent is quite sensitive to light, and under light, it is easy to induce photochemical reactions, resulting in structural changes. Therefore, it should be stored in a dark container, such as a brown glass bottle, or in a dark storage cabinet.
Fourth, when storing, pay attention to isolation from other chemicals. Due to its unique chemical properties, if mixed with incompatible chemicals, chemical reactions may occur, resulting in dangerous conditions. Be sure to store them according to the nature of the chemicals.
Finally, the storage place should be kept well ventilated. If the air in the storage environment is not ventilated, once the reagent evaporates, the accumulated gas may pose a safety hazard. Good ventilation can disperse the volatile gas in time and maintain the safety of the storage environment. Only with such meticulous care can 3- (p-iodophenyl) -2- (p-nitrophenyl) -5-phenyltetrazole chloride be properly stored to ensure its performance and quality.
What are the potential risks or side effects of 3- (p-iodophenyl) -2- (p-nitrophenyl) -5-phenyl Tetrazolium Chloride?
3 - (p-iodophenyl) - 2 - (p-nitrophenyl) - 5 - phenyl tetrazole chloride, this substance exists in the world, but also has potential risks and side effects.
In the experimental environment, although it is a commonly used reagent, contact with this substance may be harmful. If it is accidentally touched on the skin, it can cause irritation, making the skin reddish, itchy, and even rash. If it enters the eye, it is even more harmful, or damages the eye tissue and affects the visual objects.
If it inhales its dust, it is not a good deed. Or cause irritation of the respiratory tract, make people cough, asthma, and even cause dyspnea. If accidentally ingested, it may harm the internal organs and cause gastrointestinal discomfort, such as abdominal pain, vomiting, and diarrhea.
In addition, this thing is not completely harmless in the environment. It may affect the surrounding ecology, and it may be potentially dangerous to water, soil, and organisms. Therefore, when using this thing, it is necessary to follow strict procedures and take protective measures to avoid endangering life and protect yourself and the environment.
