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4-iodonitrotetrazolium what is Violet?
4 - iodonitrotetrazolium Violet is a chemical substance. It is a purple dye and is widely used in many fields such as biochemistry and medicine.
This substance is often used as a color reagent. It plays a key role in the detection of cell viability and proliferation. When the cell is viable, the intracellular dehydrogenase can reduce 4 - iodonitrotetrazolium Violet to produce an insoluble nail product. This product is purple, and its absorbance can be measured by a specific instrument to accurately evaluate the activity of the cell.
Furthermore, in microbiological research, 4 - iodonitrotetrazolium Violet is also used. It can be used to identify the respiratory activity of microorganisms, and to infer the metabolic characteristics of microorganisms by observing their reduction status.
Its chemical structure is unique and composed of specific atoms. This structure gives it special chemical and physical properties, enabling it to function effectively in the above various experiments and research scenarios, helping researchers to further explore the relevant characteristics and behaviors of cells and microorganisms.
What are the main uses of 4-iodonitrotetrazolium Violet?
4-Iodinitrotetrazolium violet, that is, 4-iodonitrotetrazolium Violet, its main uses are as follows:
In the field of biochemical experiments, it is often used as a redox indicator. Because of the variable color of reducing agents, it can clearly show the redox reaction process in organisms. For example, when studying cell respiration, the color change can be used to measure the activity of mitochondrial electron transfer chain, and then the respiratory and metabolic status of cells can be known.
In microbial detection, there are also many applications. It can be used to detect the redox ability of microorganisms such as bacteria to substrates, and help researchers judge the type and activity of microbial metabolism. For example, when identifying some special bacteria, it is used as an indicator to observe the color change after the bacteria metabolize specific substances, so as to
In plant physiology research, 4-iodonitrotetrazolium violet also plays an important role. It can detect changes in the redox state of plant tissues or cells under stress, such as drought, salinity and other stresses, and use its color response to know the response of plant antioxidant system and the degree of oxidative damage to cells, providing a key basis for revealing the adaptation mechanism of plant stress.
How is 4-iodonitrotetrazolium Violet used?
4-Iodinitrotetrazolium violet (4-iodonitrotetrazolium Violet) is a commonly used biochemical reagent. Its usage is as follows:
This reagent is very useful in the experiment of cell viability detection. Usually, an appropriate concentration of reagent solution is prepared first, which is usually dissolved in a specific buffer, and is precisely prepared to an appropriate concentration. The concentration varies according to the experimental object and purpose.
Taking cell culture as an example, an appropriate amount of 4-iodinitrotetrazolium violet solution is added to the cultured cell system. It can penetrate into the cell and undergo a reduction reaction under the action of cell mitochondria. Vibrant cells with complete mitochondrial function can reduce 4-iodinitro-tetrazolium violet to methylbenzene products. This product is purple, and its absorbance can be measured at a specific wavelength by spectrophotometer and other instruments. According to the value of absorbance, the cell viability can be inferred.
In terms of enzyme activity determination, if the reaction of the measured enzyme is related to the redox process, 4-iodinitro-tetrazolium violet can also be used as an indicator. When an enzymatic reaction occurs, the redox state of the system changes, which affects the reduction of 4-iodinitro-tetrazolium violet. According to its color change, the enzyme activity can be observed. In the field of microbial detection, adding it to the culture medium, microorganisms with metabolic vitality can cause 4-iodinitrotetrazolium violet discoloration, which is helpful for identifying and counting microorganisms. In short, the use needs to be based on specific experimental needs, rigorous operation, and good control conditions to obtain reliable results.
4-iodonitrotetrazolium What are the precautions for using Violet?
4-Iodinitrotetrazolium violet, this is a commonly used reagent in experiments. When using it, many matters need to be paid attention to.
First, it is related to the preservation method. This reagent is quite sensitive to light and humidity, and should be stored in a dry and dark place. The temperature should also be controlled, usually refrigerated at 2-8 ° C. If it is not stored properly, such as under light or in a place with high humidity, its chemical properties are volatile, resulting in a decrease in activity, which affects the accuracy of the experimental results.
Second, when taking it, the operation must be precise and careful. Because of its certain toxicity and irritation, protective equipment such as gloves and goggles is required during the taking process to prevent contact with the skin and eyes. The tool should be clean and dry to avoid impurities mixed in and affect the purity of the reagent. When weighing, use a precise balance and take the exact amount required for the experiment. Too much or too little may make the experiment deviate from expectations.
Third, when preparing the solution, the choice of solvent is the key. Generally speaking, it is appropriate to use specific organic solvents, such as dimethyl sulfoxide (DMSO). The purity of the solvent cannot be ignored. Impure solvents or impurities may react with the reagent and change the properties of the solution. The preparation process needs to be fully stirred to ensure that the reagent is completely dissolved, otherwise the concentration of the solution will be uneven and the experimental results will be biased.
Fourth, the use environment should also be particular. The experimental site should be well ventilated to reduce the accumulation of toxic gases. The experimental table should be kept clean to avoid contact with other chemical substances and unnecessary reactions. And the experimental process should strictly follow the established procedures, and the steps and conditions should not be changed at will.
Fifth, after the experiment is completed, the remaining reagents should not be discarded at will. It should be properly handled in accordance with relevant regulations to prevent pollution of the environment. For used utensils, it is also necessary to clean them in time to remove residual reagents for subsequent use.
What is the market price for 4-iodonitrotetrazolium Violet?
4-Iodinitrotetrazolium violet, the price of this product in the market, it is difficult to generalize. The change in price is affected by various factors.
First, it is related to quality. Those with high quality are refined, high purity, rare impurities, and their price must be high; while those with lower quality, low purity, and slightly more impurities are slightly cheaper.
Second, it depends on supply and demand. If there are many people in the market, but the supply is small, in order to balance, the price will rise; conversely, if the supply exceeds the demand, the merchant will sell their goods, and the price may drop.
Third, the manufacturer is also the key. Different manufacturers have different pricing due to differences in craftsmanship, cost, and brand. Produced by famous factories, with their word-of-mouth and reputation, the price may be higher than that of ordinary manufacturers.
Fourth, the amount of quantity also affects. If the buyer buys a large batch, the manufacturer may give a discount, and the total price may drop; if the purchase quantity is small, there is no such discount, and the unit price may be high.
Under normal circumstances, if the purchase is in small quantities and the quality is acceptable, the price per gram may be between tens and hundreds of yuan; if the purchase is in large quantities, and the quality is average, the price may be slightly lower. However, this is only a rough estimate. The market situation changes. If you want to know the exact price, you need to consult the relevant suppliers and compare their quotations to obtain an accurate number.
