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What is the chemical structure of 5 '-O- (dimethoxytriphenylmethyl) -5-iodine-2' -deoxyuridine?
This is a description of an organic compound, 5% 27 - O - (diethoxy triphenyl methyl) - 5 - cyanogen - 2% 27 - deoxyadenosine. To understand its chemical structure, it is necessary to analyze the meaning of each part.
"5% 27 - O -" refers to a specific position (labeled% 27) of carbon 5, to which the oxygen atom is attached. " (diethoxy triphenyl methyl) ", this is a complex substituent. In the case of ethoxy, the ethyl group is connected to the oxygen group (-O - CH < -CH
), which has a diethoxy group; triphenyl methyl is the central carbon atom with three phenyl groups (C (C < H ₅)₃ ) , This substituent is attached to the oxygen atom of carbon 5.
"5-cyanide" indicates that there is also a cyano group attached to carbon 5 (-C ≡ N). "2% 27-deoxyadenosine", adenosine is composed of adenine linked to ribose, and 2% 27-deoxygenation indicates that there is one less hydroxyl group (-OH) on carbon 2 of ribose.
In summary, the structure of this compound is: 2% 27-deoxyadenosine as the main body, carbon No. 5 is connected with a diethoxy triphenylmethyl substituent through an oxygen atom, and carbon No. 5 is also connected with a cyanide group. Its overall structure fuses a variety of chemical groups, and may have specific properties and uses in the fields of organic synthesis and biochemistry.
What are the applications of 5 '-O- (dimethoxytriphenylmethyl) -5-iodine-2' -deoxyuridine?
5% 27-O- (diacetyltribenzyl) - 5-fluoro-2% 27-deoxyuridine, this substance is widely used. In the field of medicine, it is a good antitumor drug. Cover tumor cells proliferate rapidly and require a large number of nucleotides to synthesize DNA. The structure of this drug is similar to uracil nucleoside. It can be incorporated into the DNA synthesis process of tumor cells, but it hinders its normal synthesis, so that tumor cell growth is inhibited and proliferation is blocked, achieving anti-cancer effect.
In the context of scientific research, it is often used as a tool to explore the mechanism of DNA synthesis. Scholars can observe the DNA synthesis status of cells in the environment containing this drug, clarify the relevant molecular mechanisms and regulatory pathways, and pave the way for in-depth understanding of the mysteries of life.
And in cell biology research, it can also be used to mark specific cell populations. Because this substance can be specifically incorporated into cells undergoing DNA synthesis, it can help researchers track and identify specific cells, and gain insight into the laws of cell proliferation, differentiation and development.
This drug is an indispensable and important substance in the fields of medicine and scientific research, and has made great contributions to the development of related fields.
What is the synthesis method of 5 '-O- (dimethoxytriphenylmethyl) -5-iodine-2' -deoxyuridine?
The synthesis of 5% 27 - O- (diethoxytriphenylmethyl) - 5 - cyanogen - 2% 27 - deoxyuridine is a key issue in the field of organic synthesis. This synthesis path usually requires multiple steps of delicate reactions to achieve.
The first step often requires the preparation of suitable starting materials. Starting with specific sugar derivatives and compounds containing cyanide groups, fine reaction conditions are used to promote the clever combination of the two. In the meantime, careful selection of solvents, catalysts and temperatures may be required to ensure that the reaction proceeds in the desired direction.
Second step, diethoxytriphenylmethyl protecting group is introduced. This process requires the selection of appropriate reagents and reaction conditions according to the principle of protective group chemistry. Or in a mild alkaline environment, the nucleophilic substitution reaction between the sugar derivative and the diethoxy triphenylmethyl halide can occur to precisely generate the target protective product. The key to this step is to closely monitor the reaction process to avoid the growth of excessive reactions or side reactions.
Furthermore, for the deoxygenation process, it is often necessary to use specific reducing reagents, such as lithium aluminum hydride, to convert the hydroxyl group at a specific position on the sugar ring into hydrogen atoms in a suitable reaction system to achieve the purpose of deoxygenation. This step requires very strict reaction conditions, and factors such as moisture and temperature may have a significant impact on the reaction results.
After each step of the reaction is completed, a series of separation and purification operations are required, such as column chromatography, recrystallization, etc., to obtain a high-purity 5% 27-O- (diethoxytriphenylmethyl) - 5-cyanogen - 2% 27-deoxyuridine product. The entire synthesis process is like a carefully choreographed chemical "dance", with each step interlocking, and the control of reaction conditions and the accuracy of experimental operations are extremely demanding.
How is the purity of 5 '-O- (dimethoxytriphenylmethyl) -5-iodine-2' -deoxyuridine tested?
To determine the purity of 5% 27 - O - (diethoxytribenzoyl) - 5 - cyanogen - 2% 27 - deoxyadenosine, you can follow the following method.
The first high-performance liquid chromatography (HPLC) is recommended. This is a commonly used purity detection method in modern times. Make a suitable solution of 5% 27 - O - (diethoxytribenzoyl) - 5 - cyanogen - 2% 27 - deoxyadenosine to be tested and inject it into the HPLC instrument. According to its molecular characteristics, select a suitable chromatographic column, such as C18 column. A suitable mobile phase is prepared and pushed by a pump to separate the sample in the column. Different impurities and targets have different elution times due to their different distribution coefficients in the stationary phase and the mobile phase. The chromatographic peaks of each component are recorded by a detector, such as an ultraviolet detector. According to the proportion of peak area, the purity of the target can be calculated. If the peak shape is sharp, the separation degree is good, and there is no noise peak interference, the purity is quite high.
Furthermore, mass spectrometry (MS) can be used. This can confirm the molecular structure and purity of the compound. The sample is ionized, and the ions are separated according to the mass-to-charge ratio (m/z) under the action of electric and magnetic fields. High purity of 5% 27 - O - (diethoxytribenzoyl) - 5 - cyanogen - 2% 27 - deoxyadenosine, the mass spectrogram should show a clear molecular ion peak without excessive interference from miscellaneous peaks. If there are impurities, additional ion peaks will appear, according to which the type and approximate content of impurities can be determined to clarify the purity status.
In addition, melting point determination is also available. Pure 5% 27 - O - (diethoxytribenzoyl) - 5 - cyanogen - 2% 27 - deoxyadenosine has a specific melting point range. Measured with a melting point instrument, if the measured melting point is consistent with the literature or theoretical values, and the melting range is very narrow, such as 1-2 ° C, the purity is high. If the melting range is too wide or the melting point is deviated, there are many impurities in it, which affects the purity.
Or use nuclear magnetic resonance (NMR) method. According to the chemical environment differences of different atoms, there are different signal peaks on the NMR spectrum. The spectral signal peak of the pure product should be in line with the theoretical prediction. The position of the peak, the splitting situation, and the integrated area are all the evidence for judging the purity. If there are impurities, an additional signal peak will appear, and the impurity content can be estimated from the integrated area, thus the purity of 5% 27-O- (diethoxytribenzoyl) - 5-cyanogen - 2% 27-deoxyadenosine is known.
How stable is 5 '-O- (dimethoxytriphenylmethyl) -5-iodine-2' -deoxyuridine?
5% 27 - O - (diethoxytribenzoyl) - 5 - cyanogen - 2% 27 - deoxyadenosine, the stability of this substance is related to many aspects. The interaction of each group in its chemical structure has a profound impact on the stability. The diethoxytribenzoyl group, with its large conjugate system and steric resistance, provides protection for molecules to a certain extent, can resist external chemical attacks, and makes the molecular configuration relatively stable. The existence of 5 - cyano group, due to the strong electron absorption of cyano group, will change the distribution of molecular electron cloud, affect the reaction activity, or enhance or weaken the stability, depending on the specific reaction environment. The 2% 27 -deoxyribose part, although one less hydroxyl group, makes the overall structure of the molecule more compact, reduces the side reactions that may be caused by hydroxyl groups, and improves stability.
The external environment is also a key factor. When the temperature rises, the thermal movement of the molecule intensifies, which easily causes chemical bonds to vibrate, distort or even break, and the stability decreases; when the humidity is high, water molecules may participate in the reaction and react with the active group, such as hydrolysis, which destroys the molecular structure. The specific wavelength energy in the light or excites the electron transition of the molecule, causing it to undergo photochemical reactions, which affects the stability.
Storage and preparation methods are equally important. Appropriate storage containers and conditions, such as sealed, dry, and dark environments, can reduce external factors and maintain stability; if the preparation process conditions are improper, such as poor control of reaction temperature, time, pH, or impure products, containing unstable impurities, reduce overall stability.
Therefore, to maintain the stability of 5% 27-O- (diethoxy tribenzoyl) -5-cyanide-2% 27-deoxyadenosine, it is necessary to comprehensively consider its chemical structure characteristics, strictly control external environmental factors, and optimize storage and preparation methods.