What substance is Iodonitrotetrazolium?
Iodinitrotetrazolium blue (Iodonitrotetrazolium) is a class of substances with special chemical properties and uses. It is a tetrazolium compound and has applications in many fields.
Looking at its chemical structure, it contains iodine atoms and nitro groups, which endows it with unique chemical activity. In the field of biology, it is often used as a color reagent. When cells undergo specific physiological changes, such as changes in metabolic activity, iodine-nitro tetrazolium blue can react with it. Some enzymes in the cell can catalyze its reduction, and the original light yellow solution can react to produce insoluble purple nail products. With this color change, researchers can intuitively detect cell metabolic activity and cell respiration intensity.
In medical testing, iodinitro-tetrazolium blue also plays an important role. For example, by detecting the bactericidal function of leukocytes, by observing the reaction between leukocytes and iodinitro-tetrazolium blue, we can understand the immune activity of leukocytes and assist in the diagnosis of immune-related diseases. In plant physiology research, it can also be used to explore physiological processes such as plant cell stress response. When plants suffer from stress, cell metabolism changes, and iodinitro-tetrazolium blue can change color as a result, providing researchers with clues to plant physiology.
What are the main uses of Iodonitrotetrazolium?
Iodonitrotetrazolium is a commonly used reagent in biochemical experiments. Its main uses are quite extensive.
First, it has a significant effect in the field of enzyme activity detection. For example, it can be used to detect dehydrogenase activity. When dehydrogenase is present, the reagent can be reduced to produce a colored product. By observing and measuring the color change, the activity of dehydrogenase can be accurately determined. This is of great significance for studying the metabolic process of the body, because many metabolic reactions are closely related to dehydrogenase, so as to gain insight into the operation of metabolic pathways.
Second, it is also indispensable in the evaluation of cell viability. Living cells have a certain metabolic capacity, which can promote the reduction reaction of iodine nitro tetrazolium blue. By measuring the amount of reaction products, cell viability can be inferred. For example, in cell culture experiments, it is used to judge the survival state of cells after growth, proliferation or being affected by external factors, and to help researchers clarify the changes in cell physiology.
Third, in the field of microbial research, it also has important applications. It can identify microbial species, and different microorganisms have different reducing abilities to iodine-nitro-tetrazolium blue, which can be used for preliminary classification. At the same time, it can also monitor the growth process of microorganisms and understand the changes in their metabolic activities, providing a key basis for microbial culture and application.
In short, iodine-nitro-tetrazolium blue is an important research tool in many fields such as biomedicine and microbiology, helping researchers to deeply explore various biochemical processes and life phenomena in living organisms.
What are the physical properties of Iodonitrotetrazolium?
Iodinitrotetrazole (Iodonitrotetrazolium) is a genus of chemical reagents. Its physical properties are special. Looking at its shape, under normal conditions, it is in the shape of a fine powder, white as snow, or slightly yellowed, just like autumn frost. Touch it, the quality is very delicate, like a smooth jade powder.
Smell its gas, almost tasteless, but smell it finely, or have a slight fragrance, like the faint smell of mountains and forests, light and difficult to observe. Its solubility in water is quite considerable, and it gradually dissolves in water, just like snowflakes in spring water, which can form a clear solution.
The melting point is about 250 degrees Celsius. When the temperature gradually rises to this point, it gradually melts from the solid state, and if the ice disappears under the warm sun. The stability of this substance is still good. In case of hot topics and open flames, there is also the risk of decomposition, just like a tame beast changing when disturbed. Its density is larger than that of ordinary objects, and when placed in the hands, it feels quite solid, like holding a gold stone.
And when it is under light, it should also be noted that it is exposed for a long time or causes slight changes in its properties, just like a delicate flower cannot withstand the hot sun. All these physical properties are the key to recognizing this substance. When using them, you should carefully observe them, and do your best to avoid their risks.
Iodonitrotetrazolium what are the precautions during use?
Iodinitrotetrazolium blue (Iodonitrotetrazolium) should be used with caution in all experiments. First, this is a chemical reagent with certain toxicity and irritation, so when using it, it is necessary to wear protective equipment, such as gloves, goggles, etc., to avoid contact with the skin and eyes. If it is accidentally touched, rinse it with plenty of water as soon as possible and seek medical attention as appropriate.
Second, its dissolution and preparation need to follow precise procedures. Generally, it is dissolved in a suitable organic solvent. During the dissolution process, it should be gently stirred to promote its dissolution evenly, and pay attention to the selection and dosage of the solvent, so as not to affect its performance. After preparing a solution, it should be properly stored, usually at low temperature and protected from light, to prevent its deterioration.
Third, during the experimental operation, the amount and timing of addition are very critical. The addition dose needs to be precisely controlled according to the purpose and design of the experiment. If the dose is improper, the experimental results may be biased. The timing of addition is also related to the success or failure of the experiment. When the appropriate reaction stage is added, accurate results can be obtained.
Fourth, after the experiment is completed, the waste containing iodine nitro tetrazolium blue must be properly disposed of according to the regulations, and cannot be dumped at will to prevent environmental pollution. It needs to be collected centrally and handed over to professional institutions for disposal according to the chemical waste treatment process.
In short, the use of iodine nitro tetrazolium blue must be rigorous and meticulous from beginning to end, and follow the norms to ensure the smooth and safe experiment.
What is the market price range for Iodonitrotetrazolium?
Iodinitrotetrazolium blue (Iodonitrotetrazolium), the price in the market, it is difficult to hide. The range of its price often changes due to many reasons.
The first one, its quality is different, the price is different. If it is a high-purity, refined product, suitable for high-end scientific research experiments, its price will be high; if it is an ordinary quality, used for general experiments or industrial purposes, the price is slightly lower.
The second one, the purchase quantity is also the main reason. If you buy a large number, merchants often give it at a preferential price to promote transactions; if you only buy a small amount, the price may be higher.
Furthermore, the supply and demand relationship of the market also affects its price. If you need more supply and less supply, the price will rise; if the supply exceeds demand, the price may decline.
If there are multiple brands, the price will be different depending on the brand. Well-known brands, with their trust and quality, have high prices; new or unknown brands, for the market, the price or close to the people.
Roughly speaking, the price per gram may range from tens to hundreds of yuan. Small retail, or more than 100 yuan per gram; if wholesale in large quantities, the price may drop to tens of yuan per gram. However, this is only an approximate number, and the actual price should be subject to the current market conditions and the quotations of various merchants. To know the exact price, you must consult the suppliers in detail to compare their price and quality before you can choose a suitable choice.