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What are the main uses of Iodonitrotetrazolium Chloride?
Iodonitrotetrazolium Chloride, its main uses are as follows:
This reagent is often used in experiments in biology and medicine. It has a significant effect on the detection of cell viability and proliferation. When cells have metabolic activity, the dehydrogenase contained in them can reduce iodinitro tetrazolium blue chloride to produce insoluble purple nail products. By measuring the amount of nail products, the viability and proliferation of cells can be accurately measured.
In the field of microbiology, it is also quite useful. It can be used to detect the respiratory activity of microorganisms such as bacteria. Microorganisms with respiratory activity can promote the reduction reaction of iodinitro tetrazolium blue chloride. By observing the reaction phenomenon, it is helpful to understand the metabolic characteristics and activity of microorganisms.
is also used in plant physiology research. It can be used to evaluate the cell viability changes of plant tissues under stress conditions. For example, when plants are subject to stress such as drought and high temperature, the reduction ability of plant tissues to iodine-nitro-tetrazolium blue chloride can be detected to determine the degree of cell damage and explore the stress resistance mechanism of plants.
And it is sometimes used in immunohistochemical technology. It can be used to localize and qualitatively analyze specific antigens in tissues or cells through the color changes produced by its reaction with specific enzymes, which can help researchers to deeply understand the physiological and pathological processes in organisms.
What are the physical and chemical properties of Iodonitrotetrazolium Chloride?
Iodonitrotetrazolium Chloride is a very important chemical substance. Its physical properties are unique. Under normal conditions, it appears as an orange-yellow to red crystalline powder. This substance has good solubility, can be dissolved in water, and can also be dissolved to a certain extent in organic solvents such as ethanol.
Looking at its chemical properties, iodinitro tetraazole blue chloride has redox properties. In a specific reaction system, it can be used as a redox indicator. When the redox potential of the environment changes, its own chemical structure will change accordingly, resulting in significant changes in color. In general, under reduced conditions, the substance will be reduced to insoluble Formazan products, which makes it very useful in many fields of biological and chemical testing.
In biological experiments, it is often used to detect cell viability and enzyme activity. Because living cells have the ability to reduce the substance, the metabolic activity of cells can be inferred by observing the amount of purple Formazan products generated. In addition, in some microbiological experiments, it can also be used to identify the metabolic type of specific microorganisms, and to distinguish different microorganisms according to the difference in their reducing ability. In short, iodine-nitro-tetrazolium blue chloride plays an indispensable role in many scientific research fields due to its unique physicochemical properties.
What are the precautions for Iodonitrotetrazolium Chloride during use?
Iodinitrotetrazolium blue chloride (Iodonitrotetrazolium Chloride), in the experimental application, there are many matters to pay attention to.
First, its dissolution is quite critical. This is due to the dissolution of the substance, which is related to the follow-up experimental results. Appropriate solvents should be selected. Generally speaking, organic solvents such as dimethyl sulfoxide (DMSO) are often a good choice. When dissolving, it is necessary to slowly add and stir well to promote uniform dissolution. Beware of agglomeration, which makes it difficult to completely dissolve and affects the accuracy of the experiment.
For the second time, the storage conditions should not be underestimated. It should be stored in a cool, dry and dark place. If exposed to high temperature or strong light, it is easy to cause its decomposition and deterioration, reducing its activity. And it needs to be sealed and stored to prevent it from reacting with moisture, oxygen and other substances in the air and damaging its chemical properties.
Furthermore, during the experimental operation, the dosage must be precisely controlled. Too much or too little dosage will cause deviation in the experimental results. It is necessary to measure accurately according to the specific experimental plan and expected effect. And when mixing with other reagents, pay attention to the order of addition and mixing method to avoid abnormal chemical reactions caused by improper operation and affect the reliability of the final experimental data.
In addition, because of its certain toxicity and irritation, protective measures should be taken during operation. Experimenters need to wear experimental clothes, gloves and goggles to prevent contact with the skin or splashing into the eyes, causing damage to the body. After the experiment is completed, properly dispose of the remaining reagents and waste, follow relevant environmental regulations, and do not discard them at will to avoid polluting the environment.
What is the market price range for Iodonitrotetrazolium Chloride?
The market price range of Iodonitrotetrazolium Chloride varies depending on quality, specification, supply and demand. In the context of "Tiangong Kaiwu", although there is no exact record of this chemical, it can be compared to the pricing of the goods.
At present, if this chemical reagent is of ordinary laboratory grade, the purity is about 95%, and the price per gram may be in the tens of yuan. If the purity is higher, up to 98% or more, it is used for fine experiments or pharmaceutical research and development, and the price per gram may exceed 100 yuan. If purchased in bulk, the order of kilograms, the price will be reduced due to the large quantity, and the price will be reduced to less than tens of yuan per gram.
If the preparation process is complicated, the raw materials are scarce, or the market demand increases greatly and the supply is insufficient, the price will rise. On the contrary, if the process is refined, the raw materials are abundant, and the supply is excessive, the price may drop. The market price range of iodine nitrotetrazole chloride fluctuates, the gram constant is ten yuan to more than 100 yuan, and the batch is within ten yuan per gram or tens of yuan.
What are the advantages of Iodonitrotetrazolium Chloride compared to other similar products?
Iodonitrotetrazolium Chloride has significant advantages over other similar products.
First, the sensitivity is quite high. During the detection process, even the slightest changes in this substance can be keenly sensed and presented, just like the ancient Sinan, the breeze blows, the pointer turns flexibly, and it is difficult to escape the "eye" of the slightest change. It can accurately detect subtle changes in many biochemical reactions, making the detection results accurate.
Second, the stability is very strong. Like a sturdy fortress, it can still maintain its own stability in the face of many complex environmental factors such as external temperature and humidity, and will not be like fragile tissue paper, which will cause chaos if there is a slight environmental change, thus ensuring that the test results are not disturbed by the outside world and always remain reliable.
Third, good specificity. Just like a precise arrow, it can accurately hit the bullseye, only reacting to specific target substances, while turning a blind eye to other irrelevant substances. This way, the possibility of misjudgment is greatly reduced, and the specificity and reliability of detection are greatly improved.
Fourth, easy to operate. The operation process is simple and clear, and ordinary personnel can master it with a little learning, lowering the threshold for use and making it easier to be widely used. Fifth, the response is fast. Just like the agile cheetah, once the appropriate conditions are triggered, the reaction can be quickly started, and the detection results can be obtained in a short time, without long waiting, which greatly improves the detection efficiency. In the detection scene where every second counts, this advantage is particularly critical.
